Workshop 4 |
Phagocytes |
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3 - 5 April 2003 |
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Organizers |
Prof. G. Berton (Verona, Italy) Prof. M. Cassatella (Verona, Italy) |
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Molecular and cellular biology of phagocytes |
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Session I |
Innate immunity |
Thursday |
Chairs |
S.W. Edwards (Liverpool, UK) & A. Albini (Genova, Italy) | Abstract No. |
| 09.45 - 10.30 | Efferocytosis: phagocytic clearance of apoptotic cells and
related mechanisms for host-parasite interaction P. Henson (Denver, USA) |
116 |
| 10.30 - 10.45 | Phenotypical and functional heterogeneity of human lung macrophages F. Granata, B. Balestrieri, A. Petraroli, G. Giannatasio, P. Morabito, and M. Triggiani (Naples, Italy) |
114 |
| 10.45 - 11.00 | Expression and function of Toll like receptors in human neutrophils R. van Bruggen, D. Zweers, A.T.J. Tool, L.W. van der Laan, T.W. Kuijpers, and D. Roos (The Netherlands) |
115 |
| 11.00 - 11.15 | coffee break | |
| 11.15 - 12.00 | New insights on neutrophils in the angiogenic process A. Albini, R. Benelli, M. Morini, and D. Noonan (Genova, Italy) |
119 |
| 12.00 - 12.15 | Involvement of IRF-1 in CD95 mediated apoptosis in monocytic
cells E. Conte, L. Manzella, and A. Messina (Catania, Italy) |
117 |
| 12.15 - 12.30 | Role of the cyclin-dependent kinase inhibitor P21/waf1 in
neutrophil apoptosis: regulation by its cleavage by proteinase 3 S. Canteloup, V. Gausson, S. Durant, and V. Witko-Sarsat (Paris, France) |
118 |
| 12.30 - 12.45 | Normal G-protein-coupled receptor signaling in Syk-deficient
neutrophils and mast cells A. Mócsai, H. Zhang, Z. Jakus, J. Kitaura, T. Kawakami, and C.A. Lowell (Budapest, Hungary/ San Francisco, CA, USA/San Diego, CA, USA) |
158 |
| 12.45 - 13.00 | Modulation of human dendritic cell responses by sphingosine
1-phosphate C. Pizzirani, M. Idzko, E. Panther, S. Corinti, A. Morelli, D. Ferrari, F. Di Virgilio, G. Girolomoni, and J. Norgauer (Ferrara, Italy/Freiburg, Germany/Rome, Italy) |
148 |
| 13.00 | End of Session I | |
| Lunch / poster viewing | ||
Session II |
Phagocyte-cytokine networks |
Thursday |
| Chairs | F. Bazzoni (Verona, Italy) & D. Roos (Amsterdam, The Netherlands) | Abstract No. |
| 15.00 - 15.45 | Influencing innate and adaptive immunity by manipulating macrophage
cytokine production D. Mosser (College Park, USA) |
|
| 15.45 - 16.00 | Mechanisms involved in interleukin-15 activation of human
neutrophils M. Pelletier, C. Ratthé, and D. Girard (Québec, Canada) |
124 |
| 16.00 - 16.15 | Regulation of IL-12 p40 and p70 production by blood and alveolar
phagocytes during severe sepsis F. Ethuin, C. Delarche, M.A. Gougerot-Pocidalo, L. Jacob, and S. Chollet-Martin (Paris, France) |
122 |
| 16.00-16.15 | coffee break | |
| 16.15 - 17.00 | Role of the transcription factor STAT3 and of its isoforms
alpha and beta in mediating cytokine signalling in macrophages as well as
in other cell types V. Poli (Turin, Italy) |
|
| 17.00 - 17.15 | Inhibitory effects of interleukin 10 on macrophage activation
triggered by toll receptor agonists: role of suppressor of cytokine signaling
(SOCS)-3 C. Berlato, L. Gatto, M. Cassatella, and F. Bazzoni (Verona, Italy) |
123 |
| 17.15 - 17.30 |
Cytokine regulation in myocardial ischemic disease E. Vecile, A. Abbate, R. Cattaruzzi, G. DiSciascio, and A. Dobrina (Rome, Italy) |
125 |
| 17.30 - 17.45 | Characterization of the mechanism of action of the CXCR1 inhibitor
repertaxin E. Galliera, R. Bertini, M. Allegretti, M. Locati, A. Mantovani, and F. Colotta (Milan, Italy) |
126 |
| 18.00 | End of Session II | |
Session III |
Signal transduction |
Friday |
Chairs |
T. Andersson (Malmö, Sweden) & A. Gougerot-Pocidalo (Paris, France) | Abstract No. |
| 9.40 - 10.20 | The diverse roles of phospholipase A2 isotypes in phagocytes R. Levy (Beer-Sheva, Israel) |
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| 10.20 - 10.35 | Regulation of neutrophil chemotaxis by cyclic GMP-dependent
protein kinase 1 (cGKI) C. Werner, V. Godfrey, F. Hofmann, and K.B Pryzwansky (München, Germany/Chapel Hill, USA) |
127 |
| 10.35 - 10.50 | Survival signalling by p38-mitogen-activated protein kinase
can occur through phosphorylation of caspases M. Alvarado-Kristensson, F. Melander, and T. Andersson (Malmö, Sweden) |
128 |
| 10.50 - 11.00 | Regulation of apoptosis by Forkhead (FOXO) transcription factors P.J. Coffer, K.U. Birkenkamp, P.F. Dijkers, E.W.-F. Lam, and L. Koenderman (Utrecht, The Netherlands) |
158C |
| 11.00 - 11.15 | coffee break | |
| 11.15 - 12.00 | A retro look at tyrosine kinases and neutrophil activation P. Naccache (Lavalle, Canada) |
|
| 12.00 - 12.15 | SRC-dependent signaling is a key step in the process of autoregulation
of MAC-1 adhesive function in human PMN P. Piccardoni, L. Totani, S. Manarini, Z. Bagoly, R. Pecce, A. Piccoli, C. Cerletti, and V. Evangelista (Santa Maria Imbaro, Italy) |
129 |
| 12.15 - 12.30 | Avidity regulation of integrin CR3 (CD11b/CD18) in macrophages J. Chinegwundoh, H. Janssen, J. Calafat, and S. Greenberg (New York, USA/Amsterdam, The Netherlands) |
130 |
| 12.30 - 12.45 | Down-regulation of Rac activity during ß2 integrin-mediated
adhesion of human neutrophils K. Dib, F. Melander, L. Axelsson, M.-C. Dagher, P. Aspenström, and T. Andersson (Malmö, Sweden/Grenoble, France/Uppsala, Sweden) |
131 |
| 12.45 - 13.00 | Modulation of ß2-integrin affinity and avidity V. Patcha, J. Wigren, M. Winberg, and E. Särndahl (Linköping, Sweden) |
132 |
| 13.00 | End of Session III | |
Session IV |
Adhesion, phagocytosis and cytoskeleton dynamics |
Saturday |
Chairs |
V. Witko-Sarsat (Paris, France) & O. Stendahl (Linköping, Sweden) | Abstract No. |
| 9.40 - 10.30 | Mechanisms of inside-out control of adhesion of leukocytes
to endothelial cells L. Koenderman (Utrecht, The Netherlands) |
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| 10.30 - 10.45 | Regulation of macrophage movement by Fgr, Hck and Cbl E. Caveggion, S. Continolo, F. Pixley, R.E. Stanley, D.D.L. Bowtell, C.A. Lowell, and G. Berton (Verona, Italy/New York, USA/Melbourne, Australia/San Francisco, USA) |
133 |
| 10.45 - 11.00 | Inhibition of phagosomal maturation by lipophosphoglycan (LPG)
from Leishmania donovani is dependent on lipid rafts in human macrophages Å. Holm, M.Lerm, K.-E. Magnusson, A. Descoteaux, and B. Rasmusson (Linköping, Sweden/Laval, QC, Canada) |
134 |
| 11.00-11.15 | coffee break | |
| 11.15 - 12.00 | p59Hck and p61Hck play distinct roles in human phagocytes I. Maridonneau-Parini (Toulouse, France) |
|
| 12.00 - 12.15 | Chlamydia Pneumoniae binds to resting human platelets
and triggers P-selectin expression and aggregation M. Majeed, H. Johansson, and T. Bengtsson (Linköping, Sweden) |
158B |
| 12.15 - 12.30 | Detection of Mannose Binding Lectin (MBL) mediated phagocytosis K.M. Dolman, M.H. de Blauw, K.E. van Baal, A.T.J. Tool, R. van Zwieten, D. Roos, and T.W. Kuijpers (Amsterdam, The Netherlands) |
136 |
| 12.30 - 12.45 | Bacterial response to conditions in the neutrophil phagosome H. Rosen, B.J. Staudinger, M.A. Oberdoerster, P.J. Lewis, and J.R. Johnson (Minnesota, USA) |
137 |
| 12.45 | End of Session IV | |
Session V |
NADPH oxidase |
Saturday |
Chairs |
M. Markert (Lausanne, Switzerland) & S. Dusi (Verona, Italy) | Abstract No. |
| 14.15 - 15.00 | p67phox-Rac1 chimeras require both liberty and fraternity
to activate the NADPH oxidase E. Pick (Tel Aviv, Israel) |
138 |
| 15.00 - 15.15 | New PLB X+CGD cellular models for the study of
mutations' impact on NADPH oxidase activity M.J. Stasia, C. Bionda, X. J. Li, R. van Bruggen, B. Polack, D. Roos, and F. Morel (CHU Grenoble, France/Amsterdam, The Netherlands) |
139 |
| 15.15 - 15.30 | The stimulation of human monocytes with interferon-gamma induces
the phosphorylation of PU.1 and its binding to gp91phox promoter via activation
of a classical protein kinase C S. Dusi, P. Mazzi, D. Margotto, F. Wientjes*, and M. Donini (Verona, Italy/London, UK ) |
140 |
| 15.30 - 15.45 | Structural and functional analysis of the cytoplasmic domains
in gp91phox and Nox1 T.K. Epperson, A.J. Valente, K.-H. Krause, and R.A. Clark (San Antonio, TX, USA) |
158D |
| 16.00 - 16.15 | coffee break | |
| 16.15 - 17.00 | Activation of the phagocyte NADPH oxidase by phosphatidic
acid L. McPhail (Winston-Salem, USA) |
|
| 17.00- 17.15 | MRP8 and MRP14 activate NADPH oxidase via a direct interaction
with cytochrome b558 M. H. Paclet, S. Berthier, A. W. Coleman, F. Roux, S. Vergnaud, and F. Morel (Grenoble, France/Lyon, France) |
141 |
| 17.15 - 17.30 | Tosyl Phenylalanine Chloromethyl Ketone inhibits NADPH oxidase
activation by preventing p47phox phosphorylation and translocation M. Gillibert, Z. Derhy, M. Terrier, J. El Benna, and F. Lederer (Gif-sur-Yvette, France/Paris, France) |
142 |
| 17.30 - 17.45 | Absence of leukocyte glucose-6-phosphate dehydrogenase (G6PD)
does not affect the neutrophil bactericidal activity B. Wolach, M. Ashkenazi, R. Grossmann, Z. Friedman, R. Gavrieli, and D. Roos (Kfar Saba, Tel-Aviv, Israel/Amsterdam, The Netherlands) |
144 |
| 17.45 - 18.00 | Zinc-induced intra-cellular translocation of fatty-acid binding
protein p34, S100A8 and S100A9 to the membrane of human neutrophils M. Markert, R. Hotz, M. Vaglio, J. Mauël, and G. Siegenthaler (Lausanne, Switzerland/Epalinges, Switzerland) |
145 |
| 18.00 | End of workshop | |
Posters
121
G-CSF-stimulated neutrophils are a prominent source of functional BLYS
P. Scapini, B. Nardelli, G. Nadali, F. Calzetti, G. Pizzolo, and M.A. Cassatella
(Verona, Italy/Rockville, MD, USA)
135
Surfactant protein A modulates Mycobacterium tuberculosis uptake by cholesterol-depleted
human leukocytes
M. Morosini, F. Meloni, A. Marone Bianco, E. Paschetto, A. Malinverno, and A.
Fietta (Pavia, Italy)
143
Oligomannan suppresses neutrophil and monocyte respiratory burst: a possible
mechanism for granulomatous inflammation in Crohn's Disease
C.M. Mpofu, J.M. Rhodes, B.J. Campbell, and S.W. Edwards (Liverpool, UK)
146
Role of Hck and Fgr in the neutrophil response to FMLP
L. Fumagalli, C.A. Lowell, and G. Berton (Verona, Italy/San Francisco, USA)
147
Ethanol-induced inhibition of cytokine release and protein degranulation in
human neutrophils
J. Taïeb, C. Delarche, F. Ethuin, S. Selloum, T. Poynard, MA. Gougerot-Pocidalo,
and S. Chollet-Martin (Paris, France)
149
Chemokine expression by lymphatic endothelial celIs
E. Vecile, S. Mancardi, N. Dusetti, G. Stanta, O. Burrone, and A. Dobrina (Trieste,
Italy)
150
Lipophosphoglycan (LPG) from Leishmania donovani inhibits phagolysosome
fusion in human neutrophils
M. Lindmark, C. Andersson, Å. Holm, O. Stendahl, and B. Rasmusson (Linköping,
Sweden)
151
Generation of biologically active angiostatin kringle 1-3 by activated human
neutrophils
P. Scapini, and M.A. Cassatella (Verona, Italy)
152
Different recognition and scavenging of native and truncated CCL22 by the chemokine
receptor D6
R. Bonecchi, M. Locati, M. Vulcano, M. Sironi, A. Fra, M. Gobbi, A. Vecchi,
S. Sozzani, and A. Mantovani (Milan, Italy)
154
Transient decrease in neutrophil migration after aerobic exercise
R. Gavrieli, S. Geffen Ben-Dror, A. Eliakim, B. Falk, Y. Radnai, and B. Wolach
(Kfar Saba, Israel/Tel-Aviv, Israel)
155
Production of oxygen radicals in phatogen-activated dendritic cells
E. Riboldi, M. Vulcano, D. Lissandrini , P. Mazzi , S. Dusi , and S. Sozzani
(Verona, Italy/Brescia, Italy)
156
Regulation of the chemokine receptor CXCR4 by hypoxia
T. Schioppa, A. Saccani, S. Bernasconi, A. Mantovani, and A. Sica (Milan, Italy)
157
Apoptotic neutrophils in the circulation of patients with glycogen storage disease
type 1b (GSD1b)
T.W. Kuijpers, N.A. Maianski, A.T.J. Tool, G.P.A. Smit, J.P. Rake, D. Roos,
and G. Visser (Amsterdam, The Netherlands/Groningen, The Netherlands/Utrecht,
The Netherlands)
